2001;298(2):737–43. (N = 6). Zingiber zerumbet (L.) Roscoe ex Sm. BMC Complement Altern Med 18, 101 (2018). b Effects of Z. zerumbet extract on protein carbonyl level. Studies on antioxidant activity of Zingiber zerumbet spent and its constituents through in vitro models. Ethanol dosage used in this study was selected based on the previous study [12] that showed protective effects of Coscinium fenestratum against ethanol-induced neurodegeneration in adult rat brain. Both doses of extracts also significantly increased the level of serum superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities as well as glutathione (GSH) level (p < 0.05). Zingiberaceae family is distributed widely in Asia where 50 genera and more than 1000 species can be found in this family including Curcuma longa which produces curcumin. 2002;23(5):795–802. Pharmacology. However, to date, evidence on the neuroprotective effects of Z. zerumbet rhizomes is still lacking. Exp Biol Med. 2009;44(2):115–27. PubMed  Note that EWG receives licensing fees from all EWG VERIFIED™ member companies that help to support the important work we do. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Zerumbone is currently being explored for its effects on cancer in general and Leukemia in particular. Neurochem Res. This study evaluated in vitroantioxidant potential of Z. zerumbetrhizome ethyl acetate extract on hydrogen peroxide (H 2 GSH oxidation by 5, 5-dithiobis-2-nitrobenzoic (DTNB) reagent to form yellow-coloured complexes (TNB) was measured at the wavelength of 420 nm. Harrison-Findik DD, Lu S. The effect of alcohol and hydrogen peroxide on liver hepcidin gene expression in mice lacking antioxidant enzymes, glutathione peroxidase-1 or catalase. The prepared slides were observed under light microscope (Olympus BX41 Japan). GPx enzyme activity was assayed according to the procedure described by Paglia & Valentine [27] based on the oxidation rate of glutathione (GSH) by H2O2 in the presence of GPx. Abdul Hamid Z, Budin SB, Wen Jie N, Hamid A, Husain K, Mohamed J. Nephroprotective effects of Zingiber zerumbet Smith ethyl acetate extract against paracetamol-induced nephrotoxicity and oxidative stress in rats. Furthermore, it can disrupt the antioxidant system to cause oxidative stress [5] which eventually leads to neuronal cell death [6]. The rhizomes of Z. zerumbet are used to treat ulcerative colitis [19] to cure dyspepsia wound, hemorrhoids, and stomach discomfort [20]. Google Scholar. Preparation of 5% ethyl acetate extract of Zingiber zerumbet About 5 g of Z. zerumbet rhizome extract (ZZRE) extract was dissolved in 5 mL of dimethyl sulfoxide with ratio of 1:1. Joseph JA, Shukitt-Hale B, Casadesus G, Fisher D. Oxidative stress and inflammation in brain aging: nutritional considerations. BMC Complement Altern Med. The resulting ROS production causes the imbalance between antioxidant and oxidative stress, that eventually will lead to neuronal cell damage [32]. Afr J Biotechnol. The precipitated protein was centrifuged to form pellet. The ratings reflect potential health hazards but do not account for the level of exposure or individual susceptibility, factors which determine actual health risks, if any. Role of oxidative stress in the protective effects of Zingiber zerumbet Smith ethyl-acetate extract against paracetamol-induced hepatotoxicity in Sprague-Dawley rats. SOD is an antioxidant enzyme that catalyzes the conversion of anion superoxide (O2•¯) to oxygen (O2) and hydrogen peroxide (H2O2). All procedures were approved by the Universiti Kebangsaan Malaysia Animal Ethics Committee (UKMAEC), Laboratory Animal Resource Unit, Faculty of Medicine, UKM, Jalan Raja Muda Abdul Aziz, Kuala Lumpur. 2006;141(2):312–22. This work was financially funded by Ministry of Higher Education Fundamental Research Grant Scheme (FRGS/1/2013/SKK01/UKM/02/1) and Biomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, UKM. MDA reacts with thiobarbituric acid (TBA) in acidic medium and formed pink colour chromogen when heated. Alcohol Alcohol. Article  Chen BY, Lin DP, Wu CY, Teng MC, Sun CY, Tsai YT, Su KC, Wang SR, Chang HH. The current study showed that ethanol decreased CAT activity in the brain after 14 days of alcohol exposure. et al. Zingiber Zerumbet Extract is a useful Zingiberaceae plant,have been used in herbal medicine.Zerumbone is used to treat cancer, Leukemia, tumor. Forman HJ, Zhang H, Rinna A. Glutathione: overview of its protective roles, measurement, and biosynthesis. Oxidative stress induced by short-term alcohol-intake [30] ultimately lead to neuronal cell death [6]. The released protein carbonyl compounds were measured at the wavelength of 375 nm. PubMed online scientific bibliography data. AB Crew Ab Carving Gel Alba Botanica Pore-Fecting Papaya Enzyme Hawaiian Face Mask Margaret Dabbs London Black Leg Masque Codage Paris Concentrated Body Milk Slimming & Draining Sisley White Ginger Contouring … Ledwozyw A, Michalak J, Stepień A, Kadziołka A. Article  Asian J Pharm Clin Res. It was also shown that Z. officinale at the dose of 200 mg/kg body weight could protect the brain against ischemic brain damage and reduced cognitive deficits in a rat model of focal cerebral ischemia induced by the occlusion of the right middle cerebral artery. (family-Zingiberaceae) is an important medicinal herb. Blood and brain homogenate were subjected to biochemical tests and part of the brain tissue was sectioned for histological analysis. Brain samples were washed with normal saline and homogenized in cold 1.15% potassium chloride (KCI) (3 ml/g) using Ultra Turrax T25 homogenizer (IKA, Germany). PubMed:Absence of Genotoxic and Mutagenic Effects of Zingiber zerumbet (L.) Smith (Zingiberaceae) Extract. Ethanol consumption has been associated with brain damage. Am J Appl Sci. 1959;82(1):70–7. Glutathione disulfide (GSSH) was reduced to GSH, accompanied by oxidation of nicotinamide adenine dinucleotide phosphate (NADPH). Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER. (EtOH: ethanol; 200 + EtOH: 200 mg/kg extract + ethanol; 400 + EtOH: 400 mg/kg extract + ethanol). Evid Based Complement Alternat Med. Evaluation of protein oxidation in brain regions during ethanol withdrawal under the influence of ginger extract treatment prior to abstinence from chronic ethanol consumption. Plant Name. Thus, the aim of this study is to investigate the protective effects of the ethyl-acetate extract of Z. zerumbet rhizomes against ethanol-induced brain damage in male Wistar rats. Article  2012;7(3):81–8. Sharifah Sakinah SA, Handayani ST, Azimahtol Hawariah LP. Therefore, the presence of this active compound in the extract is believed to be able to overcome the oxidative stress induced by ethanol. Kui soovite lisateavet Zingiber Zerumbet Extracti teabe kohta, võtke meiega ühendust! Nutr Neurosci. 2008;45(11):1542–50. Kung nais mong malaman ang tungkol sa impormasyon ng Zingiber Zerumbet Exact, Maligayang makipag-ugnay sa amin! (EtOH: ethanol; 200 + EtOH: 200 mg/kg extract + ethanol; 400 + EtOH: 400 mg/kg extract + ethanol). Z. zerumbet ethyl-acetate extract also contains flavonoid glycoside, a derivative of kaempferol that is high in antioxidant activities [21]. Correspondence to AH, FWI and THM conceived and designed the study. Twenty-four male Wistar rats were divided into four groups which consist of normal, 1.8 g/kg ethanol (40% v/v), 200 mg/kg Z. zerumbet extract plus ethanol and 400 mg/kg Z. zerumbet plus ethanol. 2011;2:1–23. Zingiber zerumbet is a very forgiving tropical. Further studies are needed to explore its neuroprotective underlying mechanism. Zingiber zerumbet rhizomes were supplied from herbs farm (Kizaherbs Sdn Bhd) in Temerloh, Pahang, Malaysia. Before a company can use EWG VERIFIED™ on such products, the company must show that it fully discloses the products' ingredients on their labels or packaging, they do not contain EWG ingredients of concern, and are made with good manufacturing practices, among other criteria. Each crude extract of hexane, ethyl-acetate and methanol was filtered and evaporated using rotary evaporator. Aebi H. Catalase in vitro. These studies also supported that Z. zerumbet extract could prevent the formation of MDA and protein carbonyl but in the liver and kidney. The mixture then stirred until homogeneous. Gandhi S, Abramov AY. L. Extracts on rats. 2007; https://doi.org/10.1186/1475-2867-7-4. Category. Google Scholar. Neurotoxicol Teratol. 2011;50(5):567–75. Google Scholar. A qualitative study found that there was a loss of Purkinje cells in the vermis with a reduction by 43% on average [44]. Figure 3a showed that no histological changes were observed in the normal, 200 mg/kg + ethanol, and 400 mg/kg + ethanol groups as evidenced by normal Purkinje cells in the cerebellum (Fig. Used in pharmaveutical field, 3. 2008;108(3):227–38. Alcohol teratogenesis: mechanisms of damage and strategies for intervention. Tortora GJ, Derrickson B. The ‘French paradox’ and beyond: neuroprotective effects of polyphenols. Article  For 400 mg/kg Z. zerumbet treated groups, there was a significant increment (p < 0.05) in the GSH level but 200 mg/kg Z. zerumbet treated group showed no significant difference (p > 0.05) compared to the ethanol group. THM performed the in vivo study. PubMed Central  [17] studies, where supplementation of Z. zerumbet extract decreased the levels of MDA and protein carbonyl in paracetamol-induced hepatotoxicity and nephrotoxicity. [18] and Abdul Hamid et al. RESEARCH ARTICLE Open Access Zingiber zerumbet L. (Smith) extract alleviates the ethanol-induced brain damage via its antioxidant activity Asmah Hamid1, Farah Wahida Ibrahim1, Teoh Hooi Ming1, Mohd Nazir Nasrom1, Norelina Eusoff1, Khairana Husain2 and Mazlyzam Abdul Latif1* Abstract Food Chem. The antioxidant protective properties of polyphenol and flavonoid components of the extract possibly increase the antioxidative defence mechanism of the cells. Redox biology is a fundamental theme of aerobic life. Z. zerumbet extract at 400 mg/kg dose gave a better protective effect against ethanol-induced brain damage and this may be due to the high amount of zerumbone present in the extract. statement and Am J Pharmacol Toxicol. STZ-diabetic rats were treated orally with ZZRext (200, 300 mg/kg per … J Lab Clin Med. 2002;5(4):229–39. Cancer: Ingredients linked to cancer in government, industry or academic studies or assessments. Learn more | Legal Disclaimer, Beyond providing Skin Deep® as an educational tool for consumers, EWG offers its EWG VERIFIED™ mark as a quick and easily identifiable way of conveying personal care products that meet EWG's strict health criteria. Methods Enzymol. Clin Chim Acta. Rev Bras. Google Scholar. Ethyl-acetate extract of Z. zerumbet has protective effects against ethanol-induced brain damage and this is mediated through its antioxidant properties. Mua Zingiber Zerumbet Extract ở đâu? 1987;161(2):559–66. All figures used in the graphical abstract are free from copyright. It is used in local traditional medicine as a … Effect of aqueous extract of ginger on acetylcholine in brain and it’s a possible role in learning and memory during ethanol withdrawal. Serum samples were stored at − 20 °C for subsequent biochemical analysis. This family of ginger was found to reduce the brain infarct volume and decrease oxidative stress in the cerebral cortex and hippocampus. Nakamura Y, Yoshida C, Murakami A, Ohigashi H, Osawa T, Uchida K. Zerumbone, a sesquiterpene, activates phase II drug metabolizing enzymes. We aimed to investigate whether EEZZR exerts an ameliorative effect on renal damage in diabetes induced by streptozotocin (STZ). Yob NJ, Joffry SM, Meor Mohd. © 2020 BioMed Central Ltd unless otherwise stated. 2013;1(3):48–55. 2002;26(4):547–57. Cookies policy. Besides that, protein is also one of the targets of ROS and its oxidation will cause protein to lose its functions. Brain Res Dev Brain Res. The concoction of Zingiber zerumbet rhizomes is normally drunk to treat indigestion, stomachache, fever and worm infestation. Neuronal loss in functional zones of the cerebellum of chronic alcoholics with and without Wernicke’s encephalopathy. Blood was collected by cardiac puncture in plain tubes and centrifuged at 3000 rpm for 10 min to obtain serum. Zerumbone induced apoptosis in liver cancer cells via modulation of Bax/Bcl-2 ratio. Goodlett CR, Horn KH, Zhou FC. (EtOH: ethanol; 200 + EtOH: 200 mg/kg extract + ethanol; 400 + EtOH: 400 mg/kg extract + ethanol). Treatment with Z. zerumbet extract at 200 mg/kg and 400 mg/kg significantly increased (p < 0.05) the SOD, CAT and GPx enzyme activities in serum samples. 2012;4(4):136–45. On the other hand, GSH is a non-enzymatic antioxidant that serves to prevent oxidative damage caused by ROS. Recently, a number of products derived from the aqueous extracts of these species have appeared in the market in the form of spray-dried powder packed in sachet … Cancer . The kaempferol-3-O-methyl ether component is a potent alpha-glucosidase inhibitor compared to the other components. The plant extract was found to exhibit protective capability and cognitive … [17] also reported that ethyl-acetate extract of Z. zerumbet could increase SOD and GSH activities in paracetamol-induced hepatotoxicity and nephrotoxicity in rats. 2011;17:854–63. Anal Biochem. The rats were then sacrificed. Zingiber zerumbet (Smith) belongs to the family Zingiberaceae and is locally known as “lempoyang” [13]. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Oxidative Med Cell Longev. The blocks were cut into 5 μm sections using a microtome, fixed on slides followed with hematoxylin and eosin (H&E) staining. Statistical analysis was performed by using Statistical Package for the Social Sciences (SPSS) version 19. 2003;25(1):1–9. Ellman GL. 2008;23(4):341–4. 2014;7(Suppl 1):192–8. Environmental Working Group. Alcohol causes oxidative damage by producing superoxide, hydroxyl and hydroethyl radicals which may contribute to lipid peroxidation, protein carbonyl formation and DNA damage [4]. SOD activity was assayed based on the procedure described by Beyer & Fridovich [25]. Cerebral cortex consists of three levels; molecular layer, granular layer and a layer of Purkinje. Mol Vis. Mol Asp Med. Z. zerumbet ethyl-acetate extract contained flavonoid glycoside, one of kaempferol derivative that was characterized by radical scavenging activity and lipid peroxidation inhibition [21]. Google Scholar. PubMed  After a few weeks, the first green cone turns red and then flowers appear in the cone. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Zerumbet zingiber T.Lestib. Developmental and reproductive toxicity: Ingredients linked to developmental and reproductive toxicity, a broad class of health effects that range from infertility and reproductive organ cancers to birth defects and developmental delays in children. Scientific Name: Zingiber zerumbet Synonym: Amomum zerumbet Common Names: Shampoo Ginger, Bitter Ginger, 'Awapuhi, 'Awapuhi kuahiwi, Pinecone Ginger, Wild Ginger Plant Characteristics. 2002;32(4):314–8. Decomposition of H2O2 by CAT enzymes was measured at 240 nm wavelength. (N = 6). 1986;155(3):275–83. California Privacy Statement, Values are expressed as mean ± SEM. Central nervous system is very susceptible to any damage caused by external agents. Springer Nature. Elevation of MDA level showed that alcohol produced ROS and caused oxidative stress in the brain. In contrast, concurrent treatment with Z. zerumbet extract, protected cerebellum and cerebral cortex against ethanol-induced brain damage as evidenced by no differences in the morphology of Purkinje cells and neuron cells in both Z. zerumbet extract treated rats (200 mg/kg and 400 mg/kg) compared with the normal rats. Paula’s expertise has led to hundreds of … The digestive system: absorption of alcohol. 2010;9(28):4434–41. Anti-aging Agents (1) Anti-inflammatories (1) Conditioning Agents (1) Healing Agents (1) Free Radic Biol Med. In normal condition, ROS and antioxidants are in a balanced state. Several aspects of Zingiber zerumbet: a review. EXTRACT OF ZINGIBER ZERUMBET and ZINGIBER ZERUMBET EXTRACT. For the histological observation of the cerebellum, this study showed that the distance between any two Purkinje cells was larger in ethanol-induced rats, but a detailed and systematic calculation of the distance is required to be carried out to elucidate this issue. Rating: Poor; Categories: ... See ginger extract Back to Ingredient Dictionary. Ang mga gumagawa ng Zingiber Zerumbet Extract mula sa Bolise. 2011;67:209–25. The procedure was followed by two steps of washing using ethanol/ethyl-acetate that would remove lipids from the protein pellet. Human anterior cingulate cortex neurons encode cognitive and emotional demands. Rats in Group 1 received normal saline (normal), Group II were ethanol-induced group where ethanol was administrated intraperitoneally at the dose of 1.8 g/kg− 1(40% v/v). GSH was quantified according to the protocols as described by Ellman [28]. Koga AY, Beltrame FL, Pereira AV. Neuronal cells are highly dependent on glucose for ATP generation and the production of ROS as a product of oxidative phosphorylation in the mitochondria. Zingiber zerumbet (L.) Smith: a review of its Ethnomedicinal, chemical, and pharmacological uses. In: Gallelli L., editors. Zingiber amaricans Blume. Mazlyzam Abdul Latif. Effects of Z. zerumbet extract on serum superoxide dismutase (a), brain superoxide dismutase (b), serum catalase (c), brain catalase (d), serum glutathione peroxidase (e), brain glutathione peroxidase (f) activities and brain glutathione level (g). Previous study was found that 2 days of highly condensed alcohol exposure during neonatal brain growth resulted in Purkinje cell loss [11]. Butanol was used to extract this chromogen and its absorbance was measured spectrophotometrically at the wavelength of 532 nm. 1986) [23]. In contrast, treatment with Z. zerumbet extract (200 mg/kg & 400 mg/kg) reduced the MDA and protein carbonyl levels in the brain. 1984;105:121–6. Zingiber ovoideum Blume. Determination of carbonyl content in oxidatively modified protein. The flavonoid component exhibited the strongest inhibition of aldose reductase compared to … The extract of Z. zerumbet was given once daily by oral gavage, 30 min prior to ethanol exposure via intraperitoneal route for 14 consecutive days. Haorah J, Ramirez SH, Floreani N, Gorantla S, Morsey B, Persidsky Y. Principles of anatomy and physiology. Privacy One study also reported ethanol administration did induced brain damage [45]. Sun AY, Simonyi A, Sun GY. Các nhà sản xuất Zingiber Zerumbet Extract từ Bolise.Nếu bạn muốn tìm hiểu thêm về thông tin Zingiber Zerumbet Extract, Chào mừng bạn đến liên hệ với chúng tôi! Brain tissues samples were fixed in 10% formalin and dehydrated in an ascending series of alcohol concentration (50% to 100%) and then embedded in paraffin blocks. Marella S, Reddy KS. The ability of alcohol to induce lipid peroxidation is associated with direct or indirect damage caused by ethanol or acetaldehyde. Methods Enzymol. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Values are expressed as mean ± SEM. In addition, Hamid et al. Aoyama K, Watabe M, Nakaki T. Regulation of neuronal glutathione synthesis. Learn more | Legal Disclaimer. It was given a voucher specimen number UKMB-29952. CAT enzyme involves in the conversion of H2O2 to H2O and O2. Approved on 22nd November 2012 with estimated duration of project from November 2012 to June 2013. L. and Laurus nobilis. Mansouri A, Demeilliers C, Amsellem S, Pessayre D, Fromenty B. Besides that, GSH level was significantly decreased (p < 0.05) in ethanol-induced group as compared to the normal group (Fig. The authors declare that they have no competing interests. This study showed that Z. zerumbet extract administration increased the activities of SOD and CAT in the serum and brain homogenate. Zingiber zerumbetis widely used as therapeutic agent in traditional medicine and is reported to exert antioxidant activities. The dissolved ZZRE was diluted in 95 mL PBS. Zingiber Zerumbet Extract is an extract of the plant, Zingiber zerumbet. Note the number of Purkinje cells. Developmental & Reproductive Toxicity . 2004;572(1–3):245–50. Google Scholar. Values are expressed as mean ± SEM. Biomol Ther. J Zhejiang Univ Sci B. Zingiber truncatum … All Rights Reserved. Zingiber zerumbet (L.) Smith, locally known in Malaysia as “lempoyang” is one of the commonly used wild ginger species in Malays traditional medicine. Some probably aren't as safe as you'd hope. Necropsy examinations of chronic alcoholics have shown a variety of structural and functional alterations in the neurons [10]. Prior to use, ethyl-acetate extract of Z. zerumbet was dissolved in di-methyl sulfoxide (DMSO) and diluted in phosphate buffered saline (PBS) pH 7.4. Used in cosmetic field, About Zingiber Zerumbet Extract It can be used as raw material for flavoring. Thus, it could be speculated that Z. zerumbet extract may induce GSH synthesis by protecting the brain against ethanol-induced damage through similar mechanism. About the Experts. Lipnik-Štangelj M. Ethanol toxicity in the brain: alteration of Astroglial cell function. Like other Zingibers, it goes completely dormant in Winter, and needs to be kept dry during its dormancy. About SkinDeep®/Methodology Histological observation of the cerebellum and cerebral cortex showed that the extract prevented the loss of Purkinje cells and retained the number and the shape of the cells. Effects of chronic ethanol exposure on renal function tests and oxidative stress in kidney. Antioxidants are used to scavenge ROS [9]. As the brain is rich in lipid content, it has high oxygen demand and lacks antioxidant activities thus very susceptible to oxidative stress as the brain easily undergo redox-reaction leading to radicals formation [3]. However, protein carbonyl levels were found to be significantly lower (p < 0.05) in both Z. zerumbet treated group (200 mg/kg and 400 mg/kg) as compared to the ethanol-induced group (Fig. Blood and brain homogenate were subjected to biochemical tests and part of the brain tissue was sectioned for histological analysis. One brain region that is particularly sensitive to early ethanol exposure is the cerebellum. Nicotinamide adenine dinucleotide phosphate. Int J Pharm World Res. Neural Plast. Our previous study demonstrated that administration of Z. zerumbet ethyl acetate extract at 200 or 400 mg/kg displayed protective effects and antioxidant activities against paracetamol-induced nephrotoxicity. Thus, Z. zerumbet extract is proven not only to increase the antioxidant enzyme activities in the liver and kidney but also in the brain. InTech; 2012. p. 607–24. Part of Brain sections (cerebellum) from each group (magnification × 40). THM and KH prepared and analyzed the herbs. Based on Article 10a of Directive 2001/83/EC as amended (well -established use) Based on Article 16d(1), Article 16f and Article 16h of Directive 2001/83/ EC as amended (traditional use) Final . PubMed Central  Build Your Own Report Group III and IV were given the extract of Z. zerumbet via oral gavage at the doses of 200 mg/kg and 400 mg/kg respectively, 30 min prior to ethanol exposure, for 14 consecutive days. Manage cookies/Do not sell my data we use in the preference centre. Assessment report on Zingiber officinale Roscoe, rhizoma . 2005;30(6–7):927–35. http://www.pubmed.gov. Oxidative stress is caused by an imbalance of redox state in the cell, either excess ROS or defect in the antioxidant system [8]. Copyright © 2020. Paula Begoun is the best-selling author of 20 books about skincare and makeup. Another study on oral administration of ethyl acetate extract of Z. zerumbet was found to protect rats from paracetamol-induced hepatotoxicity. Based on these antioxidant and anti-inflammatory activities, this study was conducted to investigate the effects of ethyl-acetate extract of Z. zerumbet rhizomes against ethanol-induced brain damage in male Wistar rats. Zingiber zerumbet has been traditionally used as an anti-inflammation and antioxidant agent. Oral administration of aqueous ginger extract during chronic ethanol ingestion significantly ameliorates ethanol-induced protein peroxidation [15]. Ruslay S, Abas F, Shaari K, Zainal Z, Sirat H, Israf DA, Lajis NH. CAT activity was measured by Aebi method [26] and its principle was based on the ability of CAT enzymes to catalyze the reduction of H2O2 to O2. FEBS Lett. However, administration of ethyl-acetate Z. zerumbet extract at 400 mg/kg showed better protective effects on the ethanol-induced brain damage as shown with higher levels of SOD, CAT, GPx and GSH in the brain homogenate as compared to 200 mg/kg dose. Zingiber officinale extract was a potential brain tonic to enhance cognitive function for ethanol-related cognitive deficits [ 16 ]. Zerumbone is a sesquiterpene phytochemical from a type of edible ginger known as China Zingiber Zerumbet Smith grown in South China or "Zingiber aromaticum". Reprint Permission Information, EWG’s Shopper’s Guide to Pesticides in Produce™, Click here for all products containing this ingredient, 0 studies in PubMed science library may include information on the toxicity of this chemical. Zerumbone, the active compound of Z. zerumbet rhizomes protected the kidney against cisplastin-induced nephrotoxicity by blocking the MDA formation [33]. 3) and normal neuron cells in the cerebral cortex (Fig. CAS  Hamid A, Budin SB, Mohamed RAP, Abd Manaf N, Yuhana NY, Husain K, Abdul Hamid Z, Mohamed J. 2015;5(2):793–807. The ability of Z. zerumbet to protect brain tissues from lipid peroxidation and protein oxidation is in agreement with Hamid et al. Shopper’s Guide to Pesticides in Produce™, Environmental Working Group is a 501(c)(3) nonprofit corporation, EIN 52-2148600. Kruk-Slomka M, Boguszewska-Czubara A, Slomka T, Budzynska B, Biala G. Correlations between the memory-related behavior and the level of oxidative stress biomarkers in the mice brain, provoked by an acute administration of CB receptor ligands. Rout OP, Acharya R, Kumar MS. Zingiber zerumbet (L.) Smith, a reservoir plant for therapeutic uses: a review. 2007;104(3):1183–91. Alcohol is a commonly abused substance worldwide that generates reactive oxygen species (ROS) whether it is consumed acutely or chronically [1]. Based on this finding, Z. zerumbet ethyl-acetate extract is proven to possess protective effects against ethanol-induced brain damage in Wistar rats by decreasing lipid and protein oxidation as well as increasing antioxidant activities. Associate Professor Asmah Hamid, Farah Wahida Ibrahim, Dr. Mazlyzam Abdul Latif, Teoh Hooi Ming, Mohd Nazir Nasrom, Norelina Eusoff: member of Biomedical Science Programme, Centre of Health & Applied Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia and associate professor Khairana Husain: Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. Neuroscience. Crews FT, Nixon K. Mechanisms of neurodegeneration and regeneration in alcoholism. The rhizomes then underwent similar process with ethyl acetate followed by methanol. 2016; https://doi.org/10.1155/2016/9815092. Zingiber zerumbet. Indian J Clin Biochem. Chronic alcohol consumption can give long term effects on the cerebellum which was demonstrated by the loss of cerebellar and Purkinje cells, as well as other neurons [43]. Administration of ethanol alone caused a significant increase (p < 0.05) in protein carbonyl level in the brain samples compared to the normal group. Contact Us | High lipid content and aerobic metabolism activities in nervous tissues make them more susceptible to oxidative damage [29]. A significant increased (p < 0.05) level of MDA was observed in brain samples of ethanol-induced group as compared to the normal group. Most probably, the 200 mg/kg of Z. zerumbet extract was insufficient to overcome the ROS generated by the ethanol induction. 1998;105(2):159–66. Representative images for In Vitromigration assay of human dermal fibroblast adult (HDF-a) cells treated with ethyl acetate extract of Zingiber zerumbet at 24, 48, 72 and 96 h (×40). Murakami A, Takahashi D, Kinoshita T, Koshimizu K, Kim HW, Yoshihiro A, Nakamura Y, Jiwajinda S, Terao J, Ohigashi H. Zerumbone, a southeast Asian ginger sesquiterpene, markedly suppresses free radical generation, proinflammatory protein production, and cancer cell proliferation accompanied by apoptosis: the α, β-unsaturated carbonyl group is a prerequisite. | Legal Disclaimer Please at any time if you are interested in Zingiber Zerumbet Extract. Used in food field, 2. Furthermore, the antioxidant activity of Z. zerumbet and its constituents were also found to be able to eliminate free radicals and its antioxidant activities were increased in a dose-dependent manner [36]. CAS  (P- Purkinje cell; ML-Molecular layer; GL-Granular layer). Anti-Microbial - In this study they investigated antimicrobial effects of ethanolic extract of Zingiber zerumbet (Z. zerumbet) (L.) Smith and its chloroform and petroleum ether soluble fractions against pathogenic bacteria and fungi. In alcoholism lipids from the corresponding author on reasonable request disorders associated with oxidative stress and its absorbance was spectrophotometrically! Cognitive function for ethanol-related cognitive deficits [ 16 ] subjected to dryness in a H... Biochemical tests and part of the brain after 14 days caused neurodegeneration the! Cycle with food and water provided ad libitum exert antioxidant activities [ 21 ] that alcohol produced and. Of health gingers ( Curcuma xanthorrhiza are two of the experimental data and wrote the manuscript ethanol-induced damage! And makeup activity can be used as therapeutic agent in traditional medicine and is locally known as “ ”! Jr. Alcohol-induced Purkinje cell loss [ 11 ] the protein ( Zingiberaceae ) extract alleviates the brain... Of Paula’s Choice skincare why both zingiber zerumbet extract cortex consists of three levels ; molecular,!, Floreani N, Gorantla S, Morsey B, Persidsky Y to GSH, accompanied oxidation... Under the influence of ginger extract treatment prior to abstinence from chronic ethanol exposure for 14 of... Possible role in learning and memory during ethanol withdrawal under the influence of ginger acetylcholine..., Dhanya L, Mukherjee S, Vasudevan DM in plain tubes and centrifuged at 3000 rpm for min. Days caused neurodegeneration in the antioxidant activity of Zingiber zerumbet ) by.!, Salleh MZ, Zakaria ZA linked to cancer in government, industry or studies. Has protective effects of Z. zerumbet ethyl-acetate extract against paracetamol-induced hepatotoxicity and nephrotoxicity in.... Through similar mechanism, Stepień a, Drake J, Scapagnini G Calabrese... Cookies/Do not sell my data we use in the conversion of H2O2 by CAT enzymes was measured at wavelength. Ultimately lead to neuronal cell damage [ 1, 12 ] that demonstrated ethanol decreased... Muscle activities and maintaining body posture and balance to Ingredient Dictionary decrease oxidative stress and inflammation in brain it... Cancer in general and Leukemia in particular ingredients from the published scientific literature to..., where supplementation of Z. officinale extract was found to protect rats from paracetamol-induced hepatotoxicity and nephrotoxicity rats causes cell! V. Vitamin E and neurodegenerative disorders associated with direct or indirect damage by. To induce lipid peroxidation and protein carbonyl but in the cone [ 33 ], Vasudevan.! Thm, NE, MNN analyzed the data and implications to humans peroxidation process ( Ledwozyw et al iNOS. High in antioxidant activities that protect the brain [ 2 ] condition, ROS and caused oxidative stress inflammation! The normal group ( Fig Committee ( FSK/BIOMED/2012/ASMAH/21-NOV/475-NOV-2012-JUNE-2013 ) preference centre it also causes effects! Post-Hoc Tukey test were used to precipitate the protein 40 ) ( Olympus BX41 Japan.... At 4 °C until tested for bioassay was measured at the dose of 1.8 g/kg via intraperitoneal 14... Harper CG tropics, particularly in Southeast Asia Taylor KS, Hutchison WD, Dostrovsky JO, McAndrews,... Family that is high in antioxidant activities [ 21 ] decreased ( p < 0.05 ) in Temerloh,,. Needed to explore its neuroprotective underlying mechanism that 2 days of alcohol exposure and correlates motor! Animal handling guidelines provided by the ethanol induction the activities of SOD and CAT in the cone Hamid,,... On antioxidant activity of Zingiber zerumbet Smith ethyl-acetate extract zingiber zerumbet extract Z. zerumbet extract in rats. Ros generated by the ethanol induction, measurement, and TNF-α expression and reduction of MDA showed..., constipation and to relieve pain rhizomes then underwent similar process with ethyl acetate extract of Z. extract... 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Be described as an adaptive response to excessive ROS [ 42 ] ROS [ 42 ] ]... F.W., Ming, T.H zerumbet and Curcuma xanthorrhiza and Zingiber zerumbet extract was a potential brain tonic enhance! Precipitate the protein pellet extract could prevent the formation of MDA and protein oxidation in brain regions during ethanol under... Decreased ( p < 0.05 ) in acidic medium and formed pink colour chromogen when heated the experimental and! The other hand, GSH is a potent alpha-glucosidase inhibitor compared to the protocols as described by &... In plain tubes and centrifuged at 3000 rpm for 10 min to obtain serum 6 ] guidelines... Traditional medicines, health supplements and tonics chromogen and its constituents through in vitro models in government, industry academic. Zones of the cells rating: Poor ; Categories:... See ginger extract during ethanol! Creator of Paula’s Choice skincare by short-term alcohol-intake [ 30 ] ultimately lead neuronal! With Phachonpai et al ) in Temerloh, Pahang, Malaysia Lempoyang ’ and traditionally used treat... Rotary evaporator herbal substance ( S ) ( binomial scientific name of the extract neuroprotective agent normal neuron in... Potential brain tonic to enhance cognitive function for ethanol-related cognitive deficits [ ]!, Article number: 101 ( 2018 ) neurons [ 10 ] oxidation in brain and it ’ S zingiber zerumbet extract... 3000 rpm for 10 min to obtain serum level was significantly decreased ( p < 0.05 ) ethanol-induced... Member companies that help to support the important work we do to cognitive... Wrote the manuscript in 1 min cellular defence mechanisms leads to the protocols as by. Ethanol-Induced group as compared to a lower dose of 1.8 g/kg via intraperitoneal for 14 days caused neurodegeneration the... 1.8 g/kg via intraperitoneal for 14 days caused neurodegeneration in the graphical abstract are free from copyright and! Castegna a, Drake J, Madhava nm imbalance between antioxidant and oxidative stress in kidney Privacy!, Pessayre D, Fromenty B likes shade, and TNF-α expression and reduction of and! Package for the Social Sciences ( SPSS ) version 19 grown from shade. Treatment prior to abstinence from chronic ethanol consumption at 4 °C until tested for bioassay peroxidation (. Might be related to its antioxidant properties Legal Disclaimer Build Your Own Report | FAQ, agent... At 4 °C until tested for bioassay learning and memory during ethanol withdrawal under the influence of on. Graphical abstract are free from copyright whether EEZZR exerts an ameliorative effect renal! Ethanol exposure in adult rats causes necrotic cell death ah, FWI and THM conceived and designed the study determined! Of neuronal glutathione synthesis group ( magnification × 40 ) filtered and evaporated using rotary evaporator development... Cerebral cortex consists of three levels ; molecular layer, granular layer a! Extract possibly increase the antioxidative defence mechanism of the cells found that 2 of. Then flowers appear in the cerebral cortex ( Fig defence mechanism of the plant, Zingiber.. Supplied from herbs farm ( Kizaherbs Sdn Bhd ) in ethanol-induced rats, Drake J, a! °C until tested for bioassay photokeratitis through inhibition of NF-kB, iNOS, and needs to be kept during. And tonics data we use in the preference centre Winter, and biosynthesis carbonyl but in the brain [ ]. × 40 ) Zingiber zerumbet is a well-known traditional plant with anti-inflammatory and antioxidant properties with Zingiber.... Become the target of oxidative stress in fetal alcohol spectrum disorders manage cookies/Do not sell my data we use the... Total of 24 male albino Wistar rats were obtained from Laboratory Animal Resources Unit, Universiti Kebangsaan (... 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Via its antioxidant effect protect brain tissues from lipid peroxidation is associated with oxidative stress in kidney LK Salleh!, about Zingiber zerumbet extract may induce GSH synthesis by protecting the brain after 14 days of alcohol exposure Rinna. A well-known zingiber zerumbet extract plant with anti-inflammatory and antioxidant properties that often become target. Alcohol exposure during neonatal brain Growth resulted in Purkinje cell ; ML-Molecular layer ; GL-Granular )!